Journal: Pharmaceutics
Article Title: Nobiletin Attenuates Inflammation and Modulates Lipid Metabolism in an In Vitro Model of Intestinal Failure-Associated Liver Disease
doi: 10.3390/pharmaceutics18010087
Figure Lengend Snippet: Effect of NOB, LPS, and INT on oxidative stress ( A ) and Nrf2 signaling pathways ( B ). ROS levels in THLE-2 cells after 24 h treatment with LPS, INT, INT+LPS, and INT+LPS+NOB (25 µM). Non-treated cells and DOXO (100 nM) served as negative and positive controls, respectively. ROS (+) and ROS (−) indicate cells with detectable or undetectable superoxide radicals, respectively. Representative histograms are shown, and data represent mean ± SEM from two independent experiments run in duplicate. Nrf2 subcellular distribution (cytosolic and nuclear fractions) and cytosolic SOD1 levels in THLE-2 cells after 24 h treatment. Representative blots are shown along with the corresponding Stain-Free™ total protein images used for total protein normalization (TPN). Lane order: (1) control, (2) LPS, (3) INT, (4) INT+LPS, (5) INT+LPS+NOB (10 µM), (6) INT+LPS+NOB (25 µM). Band intensities were quantified by densitometry and normalized to the lane-specific total protein signal obtained from Stain-Free imaging. Data are presented as mean ± SEM from two independent experiments, each performed in duplicate. Asterisks above denotes statistical significance relative to the untreated control cells with * p < 0.05, ** p < 0.01. Hashtags above denotes statistical significance relative to the INT+LPS-treated cells with # p < 0.05, ## p < 0.01, ### p < 0.001.
Article Snippet: Target proteins were detected using primary antibodies against Nrf2 and SOD1 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by AP- or HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA and BosterBio, Pleasanton, CA, USA).
Techniques: Protein-Protein interactions, Staining, Control, Imaging